fluorescence intensity fluorescence microscopes  (Nikon)

Journal: Particle and fibre toxicology

Article Title: Long-term exposure to polystyrene microplastics triggers premature testicular aging.

doi: 10.1186/s12989-023-00546-6

Figure Lengend Snippet: Fig. 3 The effect of PS-MPs exposure on oxidative stress and inflammatory response in vivo. a Determination of ROS content by CLSM in control and PS- MPs exposure group. b Quantitative analysis of ROS described in a. c Representative IFA images of 4-HNE in the testis from three groups. d Comparative quantification of 4-HNE expression level mentioned in c. e IHA images showing the measurement of p65 in the testis treated with ddH2O and PS-MPs. f Quantification of p65-positive cells described in e. g-j Representative fluorescent images showing the detection of IL-1β and IL-16 and quantitative analysis of them. Scale bars: 50 μm in e; 30 μm in a, c, g and i

Article Snippet: The cells were then washed thrice with PBS before the detection of red fluorescence intensity by CLSM (Olympus FV3000) and quantified by ImageJ software.

Techniques: In Vivo, Control, Expressing

Journal: Particle and fibre toxicology

Article Title: Long-term exposure to polystyrene microplastics triggers premature testicular aging.

doi: 10.1186/s12989-023-00546-6

Figure Lengend Snippet: Fig. 4 Characterization and internalization of PS-MPs. a PS-MPs with 1 μm particle size observed under scanning electron microscope. b Fluorescent PS- MPs with 1 μm particle size observed under CLSM. c Internalization of PS-MPs in TM4 cells. Scale bars: 30 μm in b and 50 μm in c

Article Snippet: The cells were then washed thrice with PBS before the detection of red fluorescence intensity by CLSM (Olympus FV3000) and quantified by ImageJ software.

Techniques: Microscopy

Journal: Particle and fibre toxicology

Article Title: Long-term exposure to polystyrene microplastics triggers premature testicular aging.

doi: 10.1186/s12989-023-00546-6

Figure Lengend Snippet: Fig. 6 The effect of PS-MPs exposure on oxidative stress and Ca2+ signaling in TM4 cells. a Measurement of ROS levels in TM4 cells by CLSM. b Quantitative analysis of ROS presented in a. c, d Comparative statistics of MDA and SOD content between control group and PS-MPs exposure groups. e Represen tative fluorescent images showing Ca2+ signaling after PS-MPs exposure. f Quantification of fluorescent intensity of Ca2+ signaling as presented in e. g Representative fluorescent images of Ca2+ signaling and ROS levels in TM4 cells treated with control, PS-MPs and PS-MPs + BAPTA. h, i Comparative quan tification of fluorescent intensity of Ca2+ signaling (H) and ROS (I) as presented in g. The representative images were selected from three independent and replicated experiments (n = 3). Scale bars: 30 μm

Article Snippet: The cells were then washed thrice with PBS before the detection of red fluorescence intensity by CLSM (Olympus FV3000) and quantified by ImageJ software.

Techniques: Control